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目的 探讨系统性红斑狼疮 (SLE)患者B、T淋巴细胞对SLE及正常人骨髓粒巨噬细胞集落形成单位 (GM CFU)的影响。方法 分别用SLE患者及正常人B、T及B +T淋巴细胞加入半固体甲基纤维素培养体系中 ,观察它们分别对 16例SLE患者及 12名正常人GM CFU产率的影响。结果 加入SLE患者B、T淋巴细胞后 ,分别可见 75 0 %和 37 5 %的SLE患者GM CFU形成减少 ,产率为 (10 9± 71)个 /10 5细胞和 (137± 34 )个 /10 5细胞 ,与未加淋巴细胞组比较差异均有显著性(P <0 0 1,P <0 0 5 ) ,同时加入SLE患者B和T淋巴细胞时 87 5 %的SLE患者GM CFU产率减少 ,为 (98± 31)个 /10 5细胞 ,与分别加入SLE患者B或T淋巴细胞组比较均有明显差异 (P <0 0 5 ,P <0 0 1) ;SLE患者B及B +T淋巴细胞分别对 5 8 4%、6 6 7%的正常人骨髓GM CFU形成有抑制作用 ,产率为 (2 0 3± 6 7)、(199± 92 )个 /10 5细胞 ,明显低于未加淋巴细胞组 (P均 <0 0 1) ,而正常人淋巴细胞对SLE患者GM CFU的产率与未加淋巴细胞组比较差异无显著性 (P均 >0 0 1)。结论 SLE患者B淋巴细胞无论对大部分SLE患者或大部分正常人骨髓GM CFU的形成有抑制作用 ,SLE患者的B +T淋巴细胞对SLE患者和正常人骨髓GM CFU的抑制有协同作用 ,SLE患者的B淋巴细胞的异常可能是SL
Objective To investigate the effect of B and T lymphocytes in the patients with systemic lupus erythematosus (SLE) on the granulocyte-macrophage colony forming units (GM-CSF) of SLE and normal human. Methods SLE patients and normal B, T and B + T lymphocytes were added into the semi-solid methylcellulose culture system to observe their effects on the yield of GM CFU in 16 SLE patients and 12 normal persons respectively. Results After the addition of B and T lymphocytes in SLE patients, 75.0% and 37.5% of SLE patients showed reduced GMFU formation, with a yield of (10 9 ± 71) cells / 105 cells and (137 ± 34) cells / (P <0.01, P <0.05). When adding SLE patients with B and T lymphocytes, the rate of GM CFU in 87 5% of SLE patients was significantly higher than that in non-lymphocyte group (98 ± 31) cells / 105 cells, which were significantly different from those in SLE patients with B or T lymphocytes (P <0.05, P <0.01) T lymphocytes inhibited the formation of GM CFU of human bone marrow at 58.4% and 66.7% respectively, and the yield was (203 ± 6 7) and (199 ± 92) cells / 105 cells, respectively, which were significantly lower There was no significant difference in the yield of GM CFU between normal lymphocytes and SLE patients as compared with those without lymphocytes (all P <0 01). Conclusion B lymphocytes in SLE patients inhibit the formation of GM CFU in the bone marrow of most SLE patients or most normal people. B + T lymphocytes in SLE patients have a synergistic effect on inhibition of GM CFU in SLE patients and normal people. SLE Patients with abnormal B lymphocytes may be SL