近年来对乙型肝炎抗原和胎甲蛋白的检测常用巴比妥缓冲液,离子强度为0.05,pH8.6。笔者根据电解质的导电性能,试用0.15%的氯化钠溶液,每1,000毫升溶液加1当量氢氧化钠溶液0.25毫升。琼脂板仍用巴比妥缓冲液加等量水溶化琼脂来作,孔距和厚度与原来相同,分别加好被测标本和诊断血清,两层纱布或滤纸搭桥,玻片琼脂板为8×2.5 In recent years, the detection of hepatitis B antigen and fetuin commonly used barbiturate buffer, ionic strength of 0.05, pH8.6. The author according to the conductivity of the electrolyte, try 0.15% sodium chloride solution, 1 equivalent of sodium hydroxide solution per 1,000 ml solution 0.25 ml. Agar plate is still using barbiturate buffer plus the same amount of water-soluble agar to do, the same pitch and thickness and the test specimens were added and the diagnosis of serum, two gauze or filter paper bypass, slide agar plate 8 × 2.5