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自从McCabe提出自身免疫性感觉神经性耳聋以来,国外很多学者报道用类固醇治疗此病有效。本实验通过对豚鼠脑干电位的测试,观察了Type—Ⅲ胶原引起的内耳免疫性损伤及地塞米松对感觉神经性耳聋的疗效。 1 材料和方法 Wistar远交系大白鼠、雌雄皆有,体重为200～250g,30只,随机分组每组10只。A组为单纯免疫组。将天然小牛Ⅲ型胶原(由一院中心实验室提供)溶于0.01mol/L乙酸中,用等量完全福氏佐剂乳化于大白鼠两后足掌皮下各注射50μl,共含胶原200μg,每次间隔10天,共免疫3次。B组在免疫同时给予地塞米松肌注(0.25ml/kg)末次免疫后1周再注射1次。C组为对照组,双后掌皮下各注射50μ10.01mol/
Since McCabe proposed autoimmune sensorineural deafness, many foreign scholars have reported that steroids are effective in treating this disease. In this experiment, the brain stem potential of guinea pigs was tested to observe the type III collagen-induced immune damage in the inner ear and the effect of dexamethasone on sensorineural deafness. 1 Materials and Methods Wistar outbred rats were male and female, weighing 200-250 g, and 30 rats were randomly divided into 10 groups. Group A was a simple immunization group. Natural calf type III collagen (provided by the Central Laboratory of the First Hospital) was dissolved in 0.01 mol/L acetic acid and emulsified in the same volume of complete Freund’s adjuvant in 50 μl of each mouse’s hind paws, containing a total of 200 μg of collagen. , Every 10 days, 3 immunizations. Group B was injected once more in the same week after the last immunization with dexamethasone (0.25 ml/kg). Group C is a control group and each subcutaneous injection 50μ10.01mol/