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利用11对SSR引物对5个同域分布的小果油茶和普通油茶居群进行了遗传多样性、居群遗传结构,以及种间杂交渐渗的研究.结果表明:两物种的共有等位基因数为57个,占总数的96.6%;在普通油茶中仅检测到2个特异等位基因,而在小果油茶中没有检测到特异等位基因,但两物种的等位基因频率存在一定的差别.两物种的遗传多样性均较高.在物种和居群层次上,两物种的多态信息含量的变化幅度均为0.25～0.50,属于中度多态性.同域分布的两物种间的平均遗传分化系数为0.057,明显低于物种内异域居群间的遗传分化系数的平均值(小果油茶0.332,普通油茶0.201),也低于木本被子植物种内居群间平均水平(0.102).5个同域分布物种间基因流的平均值为10.072,明显高于物种内异域间基因流的平均值(小果油茶1.006,普通油茶1.990).UPGMA聚类结果显示,小果油茶和普通油茶之间的亲缘关系非常接近,明显存在着因杂交而产生的基因渐渗.小果油茶或许是普通油茶的一个变种.
11 pairs of SSR primers were used to study the genetic diversity, population genetic structure and introgression of five populations of Camellia oleifera and Camellia oleifera with the same distribution.The results showed that the common alleles The number of which was 57, accounting for 96.6% of the total; only 2 specific alleles were detected in common Camellia oleifera, while no specific allele was detected in Camellia oleifera, but there was a certain allele frequency in both species Difference.The genetic diversity of the two species were high.At species and population level, the polymorphism information content of the two species ranged from 0.25 to 0.50, belonging to the moderate polymorphism.The homology distribution between the two species The average genetic differentiation coefficient was 0.057, which was significantly lower than the average genetic differentiation coefficient (0.332 for small fruit tea and 0.201 for common camellia oleifera) in the exotic species within the species and also lower than the average among the populations 0.102) .The mean value of gene flow among five homology-distributed species was 10.072, which was significantly higher than the average value of gene flow among different species (1.006 small-fruiting camellia and 1.990 common camellia) .UlgMA clustering results showed that small- And the common relationship between Camellia is very pick , There is obviously due to hybridization and gene introgression produced small fruit tea is probably a variant of ordinary tea.