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目的研究碳源、氮源和磷源对西洋参悬浮细胞生长,人参皂苷Re,Rb1以及西洋参多糖合成的影响。方法利用组织培养技术结合高效液相色谱法和紫外分光光度法,通过改变培养基碳源种类、氮源和磷源浓度,研究其对西洋参悬浮细胞生长,人参皂苷Re,Rb1以及西洋参多糖含量的影响。结果添加40g·L-1蔗糖的细胞干重生长率最大,加入30g·L-1蔗糖得到的人参皂苷Re、Rb1和多糖的含量最高,分别为0.531%,0.145%和3.62%。本实验保持氮源浓度60mmol·L-1,当培养基中全部为NO3-时,细胞干重生长率最低,而人参皂苷Re、Rb1和多糖含量最高,分别为对照组的2.40,1.96和4.41倍。添加5.00mmol·L-1的KH2PO4,细胞干重生长率最大。当培养基中KH2PO4浓度为0.63mmol·L-1时,人参皂苷Re、Rb1和多糖的含量最高。结论本实验确定了西洋参细胞悬浮培养的最佳碳源种类、氮源比例和磷源浓度,表明不同碳源、氮源和磷源对西洋参细胞生长和活性成分有显著影响。
Objective To study the effects of carbon source, nitrogen source and phosphorus source on the growth of suspension cells, polysaccharide synthesis of ginsenoside Re, Rb1 and American ginseng in American ginseng. Methods Tissue culture techniques were used to study the effects of different concentrations of carbon sources, nitrogen sources and phosphorus sources on the growth of suspension cells, ginsenoside Re, Rb1 and polysaccharides in American ginseng by high performance liquid chromatography and ultraviolet spectrophotometry. influences. Results The dry weight of cells with 40g · L-1 sucrose was the highest. The content of ginsenoside Re, Rb1 and polysaccharide were the highest with 0.5g / L sucrose and 0.531%, 0.145% and 3.62% respectively. In this experiment, the concentration of nitrogen source was kept at 60mmol·L-1. When all of the medium was NO3-, the cell dry weight growth rate was the lowest while that of ginsenosides Re, Rb1 and polysaccharide were the highest, which were 2.40, 1.96 and 4.41 Times Addition of KH2PO4 (5.00mmol·L-1) increased the cell dry weight. When the concentration of KH2PO4 in culture medium was 0.63mmol·L-1, the contents of ginsenoside Re, Rb1 and polysaccharide were the highest. Conclusions This study determined the optimal carbon source, nitrogen source and phosphorus source concentration in the suspension culture of American ginseng cells, indicating that different carbon, nitrogen and phosphorus sources have significant effects on the growth and active components of American ginseng cells.