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目的探讨汉黄芩素对肝癌细胞HepG2的生长抑制和诱导凋亡作用,并研究其作用的机制。方法 应用四甲基偶氮唑蓝比色法,测定汉黄芩素对HepG2细胞株活性的影响,检测其抑制肝癌细胞的剂量效应;应用形态学观察、HE染色、4’,6-二脒基-2-苯基吲哚(DAPI)荧光检测和Annexin V-PI流式检测,评价汉黄芩素对HepG2细胞的凋亡作用,并进行细胞周期的分析,蛋白质印迹法检测组蛋白去乙酰化酶1和组蛋白去乙酰化酶2蛋白表达情况。结果 汉黄芩素对HepG2细胞具有生长抑制及诱导凋亡作用,且呈剂量依赖性;并观察到细胞出现核变形、染色体凝集,出现明显的凋亡峰,细胞周期阻滞在G2/M期,蛋白质印迹法结果显示,组蛋白去乙酰化酶1和组蛋白去乙酰化酶2表达量未变化,多聚(ADP-核糖)聚合酶降解产物增加。结论汉黄芩素对肝癌HepG2细胞的增殖有抑制作用,通过阻滞细胞周期促进细胞凋亡。
Objective To investigate the effects of wogonin on the growth and apoptosis of hepatocellular carcinoma HepG2 cells and to study its mechanism. Methods MTT assay was used to determine the effect of wogonin on the activity of HepG2 cells. The effect of wogonin on HepG2 cell line was measured. The morphological changes of HEK, 2-phenylindole (DAPI) and flow cytometry with Annexin V-PI were used to evaluate the apoptosis of HepG2 cells. The apoptosis of HepG2 cells was evaluated by cell cycle analysis. Western blotting was used to detect histone deacetylase 1 and histone deacetylase 2 protein expression. Results Wogonin had inhibitory effect on HepG2 cells in a dose-dependent manner. HepG2 cells showed nuclear degeneration, chromosome condensation and obvious apoptotic peak. The cell cycle arrest was at G2 / M phase, Western blotting results showed that the expression of histone deacetylase 1 and histone deacetylase 2 did not change, and poly (ADP-ribose) polymerase degradation products increased. Conclusion Wogonin can inhibit the proliferation of HepG2 hepatocarcinoma cells and promote cell apoptosis by blocking the cell cycle.