The effects of different dietary protein and lipid levels on juvenile greenfin horse-faced filefish (Thamnaconus septen-trionalis) was experimentally evaluated in a 56-day study.Fish (initial weight 6.47 ± 0.56 g) were fed with nine different diet combi-nations containing three varying levels of protein (37％,43％,49％) and lipids (4％,7％,10％) in triplicates,where the pro-tein-to-energy (P/E) ratios of the diets varied in the range of 19.17-26.99 mgkJ-1.Results showed that fish fed diets containing 49％protein and 10％ lipid exhibited significantly lower feed intake than those fed other diets.The weight gain of fish fed diets with 43％protein was significantly higher than that of fish fed 37％ or 49％ protein diets.While no significant change in weight gain was ob-served when lipid content was increased from 4％ to 7％,weight gain decreased significantly when lipid increased from 7％ to 10％.The protein efficiency ratio and feed efficiency were highest when the fish were fed the diet with 49％ protein and 7％ lipids.Muscle crude lipid content was positively correlated with dietary protein level regardless of lipid level.The diet of 43％ protein or 7％ lipids significantly reduced the activity of alanine aminotransferase and aspartate aminotransferase in liver.The contents of aspartate ami-notransferase,superoxide dismutase and malondialdehyde were significantly increased from 4％ to 10％ lipids in plasma.The plasma total cholesterol and triglyceride contents were positively correlated with dietary lipid level at the same protein level.Our compre-hensive analysis concluded that a diet with 43％ protein and 7％ lipid,with a P/E ratio of 23.07mgkJ-1,significantly promote the growth performance,feed utilization and health of juvenile greenfin horse-faced filefish.