论文部分内容阅读
目的探讨雌二醇(E2)对Th1细胞极化以及与肝纤维化发生的影响。方法根据E2水平将47例肝纤维化患者分为A组(E2增高组)19例和B组(E2正常组)28例,阴性对照作为C组(正常人)30例。应用流式细胞术检测T细胞亚群,化学发光法检测透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原氨基末端肽(PⅢP)和Ⅳ型胶原(CⅣ);ELISA法检测干扰素-γ(IFN-γ)、白介素-2(IL-2)、白介素-4(IL-4)和白介素-10(IL-10),比较A、B组之间以及与C组的差异。结果 A组E2高于C组和B组(P<0.001)。A组HA、LN、PⅢP、CⅣ均高于C组(P<0.001,P=0.001,P=0.006,P<0.001),B组HA、LN、PⅢP、CⅣ也高于C组(均为P<0.001);A组PⅢP高于B组(P=0.045)。A组CD3+T细胞低于C组和B组(P<0.001,P=0.001),CD4+T细胞也低于C组和B组(P<0.001,P=0.024),B组CD3+、CD4+T细胞低于C组(P=0.002,P<0.001);A组、B组CD8+T细胞高于C组(P=0.001,P=0.012)。A组IFN-γ高于C组和B组(均为P<0.001),IL-2也高于C组和B组(P<0.001,P=0.003),B组高于C组(P=0.001,P=0.012);A组IL-4低于C组和B组(P=0.002,P=0.016),IL-10也低于C组和B组(P<0.001,P=0.012)。结论高E2在延缓肝纤维化形成的同时也通过Th1细胞极化影响着机体的免疫功能,加速了肝纤维化的发展,E2在肝纤维化的形成过程中扮演了双重角色。
Objective To investigate the effect of estradiol (E2) on the polarization of Th1 cells and the occurrence of hepatic fibrosis. Methods According to the level of E2, 47 patients with hepatic fibrosis were divided into group A (E2 increased group), group B (E2 normal group), group B (E2 normal group) and negative control group C (normal group). The subsets of T cells were detected by flow cytometry. The expressions of HA, LN, PⅢP and CⅣ were detected by chemiluminescence. (IFN-γ), interleukin-2 (IL-2), interleukin-4 (IL-4) and interleukin-10 (IL-10) were measured and compared between groups A and B and with group C difference. Results E2 in group A was higher than those in group C and group B (P <0.001). The levels of HA, LN, PⅢP and CⅣ in group A were higher than those in group C (P <0.001, P = 0.001, P = 0.006, P <0.001) <0.001); PⅢP in group A was higher than that in group B (P = 0.045). The CD3 + T cells in group A were lower than those in group C and B (P <0.001, P = 0.001), CD4 + T cells were also lower than those in group C and B (P <0.001, + T cells were lower than those in group C (P = 0.002, P <0.001). CD8 + T cells in group A and B were higher than those in group C (P = 0.001, P = 0.012). The level of IFN-γ in group A was higher than that in group C and B (both P <0.001), IL-2 was also higher in group A than in group C and B (P <0.001, P = 0.003) 0.001, P = 0.012). IL-4 in group A was lower than group C and group B (P = 0.002, P = 0.016). IL-10 was also lower in group A than in group C and group B (P <0.001, P = 0.012). Conclusion High E2 can delay the formation of hepatic fibrosis as well as affect the immune function of Th1 cells through polarization of Th1 cells, accelerating the development of hepatic fibrosis. E2 plays a dual role in the formation of hepatic fibrosis.