背景:现已表明,远端保护装置可减少直接经皮冠状动脉介入(PCI)时的“无复流”现象。迄今为止,尚未阐明参与冠状动脉远端保护的主要机制。目的:探讨局部存在于冠状动脉闭塞部位血中的白细胞和血小板激活状态。方法:研究纳入10例接受采用血栓栓塞保护装置及抽吸导管的直接PCI的急性心肌梗死(AMI)患者。采集冠状动脉闭塞部位以及外周股动脉血样,利用流式细胞仪检测如下指标:单核细胞和中性粒细胞表面分子;血小板表面活化抗原;白细胞-血小板结合物。 Background It has now been shown that a distal protection device can reduce the “no-reflow” phenomenon during direct percutaneous coronary intervention (PCI). To date, no major mechanism has been clarified for the protection of distal coronary arteries. Objective: To investigate the activation of leukocytes and platelets in the blood of coronary occlusion sites. Methods: We enrolled 10 patients with acute myocardial infarction (AMI) undergoing direct PCI with thromboembolic protection and aspiration catheters. Collected coronary artery occlusion sites and peripheral femoral artery blood samples, the use of flow cytometry to detect the following indicators: monocytes and neutrophils surface molecules; platelet activating antigen; leukocyte - platelet conjugate.