将来自质粒 pAD4 4 12的启动子和绿色荧光蛋白基因 gfpmut3a插入大肠杆菌 枯草芽孢杆菌穿梭载体pBE2 ,构建成芽孢杆菌表达载体 pGFP4 4 12 ,用其转化野生型生防芽孢杆菌 83 6和A 4 7等 8个菌株 ,均得到良好的发光表型。质粒稳定性实验表明重组质粒 pGFP4 4 12稳定性为 92 %。借助荧光显微镜对GFP标记的菌株A 4 7 gfp在小麦体表的定殖进行初步的研究。结果表明 :A 4 7 gfp能够在小麦根际及小麦体表定殖 (包括根表和茎叶表面 ) ;相对于在茎叶表面定殖的A 4 7 gfp在根表定殖的菌体与根的结合更为牢固 ;从根基到根尖A 4 7 gfp的定殖量有明显的减少趋势 The promoter and green fluorescent protein gene gfpmut3a from plasmid pAD4 4 12 was inserted into E. coli Bacillus subtilis shuttle vector pBE2 to construct a Bacillus expression vector pGFP4 4 12 which was used to transform wild type Bacillus pusillus 83 6 and A 4 7 8 strains, all get a good luminous phenotype. Plasmid stability experiments showed that the recombinant plasmid pGFP4 4 12 stability of 92%. Colonization of GFP-labeled strain A 4 7 gfp on the wheat body surface was studied by fluorescence microscopy. The results showed that A 4 7 gfp was able to colonize the rhizosphere and wheat body (including the root surface and the stem and leaf surface) The combination of roots is more firm; the colonization amount from the root to the root tip A 4 7 gfp has a significant decreasing trend