Gracilariopsis lemaneiformis is an economically-valued species and widely cultured in China at present.After being acclimated to different growth temperatures(15,20,25,and 30 ℃)for 7 days,the relative growth rate(RGR),nitrate reductase activity,soluble protein content and chlorophyll a fluorescence of G.lemaneiformis were examined.Results show that RGR was markedly affected by temperature especially at 20 ℃ at which G.lemaneiformis exhibited the highest effective quantum yield of PSII[Y(II)]and light-saturated electron transport rate(ETRmax),but the lowest non-photochemical quenching.Irrespective of growth temperature,the nitrate reductase activity increased with the incubation temperature from 15 to 30 ℃.In addition,the greatest nitrate reductase activity was found in the thalli grown at 20 ℃.The value of temperature coefficient Q10of alga cultured in 15 ℃ was the greatest among those of other temperatures tested.Results indicate that the optimum temperature for nitrate reductase synthesis was relatively lower than that for nitrate reductase activity,and the relationship among growth,photosynthesis,and nitrate reductase activity showed that the optimum temperature for activity of nitrate reductase in vivo assay should be the same to the optimal growth temperature.