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目的应用介入方法建立犬股骨头坏死模型。方法9只成年杂种犬全麻后,分别通过股动脉Seldinger法穿刺插管,超选一侧股骨头供血动脉行平阳霉素灌注及明胶海绵栓塞处理,建立股骨头缺血坏死模型,对侧股骨头作对照。模型犬随机分为3组,每组3只,分别于栓塞术后2、4、6周处死。所有犬均于栓塞术前及术后每周行双侧髋关节MRI扫描,栓塞术前、术后即刻、处死前行双侧股骨头供血动脉DSA造影检查,最后一次造影结束处死动物,取双侧股骨头标本送病理学观察。结果9只犬均造模成功,术后所有栓塞动脉造影复查均未见再通。造模侧股骨头在术后2周出现早期病理学改变,术后4周MRI显示股骨头坏死异常信号,病理切片出现不同程度骨细胞及骨小梁坏死,术后6周病理切片可见坏死后增生及修复反应。结论本法能成功制作出犬股骨头坏死模型,具有创伤小、造模方法简单、成模时间短、动物死亡率低等优点,是一种比较理想的造模方式。
Objective To establish a model of canine femoral head necrosis by intervention method. Methods Nine adult mongrel dogs were anesthetized with Seldinger’s femoral artery. The femoral artery was infused with pingyangmycin and gelatin sponge embolization to establish the model of femoral head necrosis. Bone as a control. The model dogs were randomly divided into 3 groups, 3 in each group, and were sacrificed at 2, 4 and 6 weeks after embolization respectively. All dogs were preoperative and postoperative bi-lateral hip MRI scans before and immediately after embolization, were sacrificed before bilateral femoral artery DSA angiography, the last time the end of angiography sacrifice animals, take bilateral shares Bone specimens sent pathology observation. Results Nine dogs were successfully established. All postoperative arterial embolization angiography did not recanalize. The pathological changes of the femoral head were observed 2 weeks after the operation. Abnormal signals of femoral head necrosis were observed by MRI at 4 weeks postoperatively. Different degrees of bone cells and trabecular bone necrosis were found in the pathological sections. After 6 weeks postoperative necrosis Hyperplasia and repair reaction. Conclusion This method can successfully produce the model of canine femoral head necrosis with advantages of less trauma, simple modeling method, shorter forming time and lower animal mortality rate. It is an ideal modeling method.