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目的:建立湖北钟祥苦荞茶中芦丁含量的HPLC-UV测定方法,测定苦荞茶中芦丁的含量。方法:HPLC-UV法,C18色谱柱(4.6 mm×150 mm,5μm),柱温:30℃,流动相:0.2%冰醋酸-甲醇(55∶45),流速:1.0mL.min-1,检测波长256 nm。结果:芦丁在0.233～1.165μg范围内,线性关系良好。平均加样回收率为99.18%。结论:此法操作简便、快速、灵敏、准确,样品处理简便易行,适于测定湖北钟祥彭墩苦荞茶中芦丁的含量。
Objective: To establish a HPLC-UV method for the determination of rutin in Bitxiang buckwheat tea in Hubei Province, and to determine the content of rutin in Tartary Buckwheat tea. Methods: The HPLC-UV method was used on a C18 column (4.6 mm × 150 mm, 5 μm) with a column temperature of 30 ° C and a mobile phase of 0.2% glacial acetic acid-methanol (55:45) at a flow rate of 1.0 mL · min- Detection wavelength of 256 nm. Results: The linear range of rutin was 0.233 ~ 1.165μg. The average recovery was 99.18%. Conclusion: The method is simple, rapid, sensitive and accurate. The method of sample processing is simple and convenient. The method is suitable for the determination of rutin in Bitter buckwheat tea.