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目的探讨内皮因子受体A(ET-A)mRNA在脑血管畸形血管中的差异性表达。方法对14例脑血管畸形和8例正常脑血管标本分别提取总RNA,应用逆转录-聚合酶链反应的方法检测ET-AmRNA的表达,然后行DNA电泳鉴定,并进行光密度扫描,半定量计算ET-AmRNA的表达。结果在脑血管畸形血管中ET-AmRNA表达水平较正常脑血管中明显下降(P<0.05)。结论脑血管畸形血管与正常脑血管中ET-AmRNA表达有差异性,为研究其在病理情况下的作用作了前期工作。
Objective To investigate the differential expression of endothelin receptor A (ET-A) mRNA in cerebrovascular malformation vessels. Methods Total RNA was extracted from 14 cases of cerebrovascular malformations and 8 cases of normal cerebral blood vessels respectively. The expression of ET-AmRNA was detected by reverse transcription polymerase chain reaction (RT-PCR), then identified by DNA electrophoresis and scanned by optical density. Semi-quantitative ET-AmRNA expression was calculated. Results The expression of ET-AmRNA in cerebrovascular malformation vessels was significantly lower than that in normal cerebrovascular vessels (P <0.05). Conclusions There is difference in the expression of ET-AmRNA between cerebrovascular malformations and normal cerebrovascular. It is a preliminary study for studying its role in pathological conditions.