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目的探讨青年女性宫颈上皮内瘤变的级别、P16和Ki67的表达与HPV感染型别的关系。方法选取2014年8月-2016年8月在该院行阴道镜活检的宫颈上皮内瘤变患者176例为研究对象,依据诊断标准分为LSIL组(99例)和HSIL组(77例),分别运用免疫组化和PCR反向杂交法检测宫颈上皮内瘤变标本中P16、Ki67的表达及HPVDNA分型。结果高危型HPV(HR-HPV)在HSIL组中的感染率明显高于LSIL组(P<0.001),P16、Ki67的阳性表达率HSIL组明显高于LSIL组(P<0.001),HPV16型感染率两组比较差异有统计学意义(P=0.02),HPV16、HPV58与P16和Ki67的表达强度有关(P<0.05)。结论随着宫颈上皮内瘤变的级别增高,P16、Ki67的表达增强;并且P16的表达强度与高危型HPV16、HPV58有关,提示临床工作中要重视这两种病毒的检测。
Objective To investigate the level of cervical intraepithelial neoplasia in young women, the relationship between the expression of P16 and Ki67 and the type of HPV infection. Methods A total of 176 patients with cervical intraepithelial neoplasia who underwent colposcopy biopsy from August 2014 to August 2016 were enrolled in this study. According to the diagnostic criteria, they were divided into LSIL group (n = 99) and HSIL group (n = 77) Immunohistochemistry and PCR reverse hybridization were used to detect the expression of P16 and Ki67 in cervical intraepithelial neoplasia and HPV DNA typing. Results The positive rate of HR-HPV in HSIL group was significantly higher than that in LSIL group (P <0.001). The positive rates of P16 and Ki67 in HSIL group were significantly higher than those in LSIL group (P <0.001) The difference between the two groups was statistically significant (P = 0.02), HPV16, HPV58 and P16 and Ki67 expression intensity (P <0.05). Conclusions With the increase of grade of cervical intraepithelial neoplasia, the expressions of P16 and Ki67 are enhanced. And the expression of P16 is associated with high-risk HPV16 and HPV58, suggesting that the detection of these two viruses should be emphasized in clinic.