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目的 :研究缺血预处理对沙土鼠脑缺血再灌注后海马羟自由基含量的影响 ,以探讨其神经保护作用的可能机制。方法 :沙土鼠前脑缺血再灌注模型 :阻断双侧颈总动脉 5min ,然后松开即再灌注。将沙土鼠随机分为假手术组 (Sh组 )、缺血组(Is组 )、短暂缺血组 (Po组 )和缺血预处理组 (Ip组 ) ,每组 6只。在动物处死前 3 0min腹腔注射水杨酸钠 10 0mg/kg。在再灌注60min ,快速断头取脑 ,在冰面上分离海马 ,用高效液相电化学检测方法测定各组沙土鼠海马 2 ,3 -二羟基苯甲酸 (2 ,3 -DH BA)、2 ,5 -二羟基苯甲酸 (2 ,5 -DHBA)的含量 (mmol/L)。结果 :沙土鼠脑缺血再灌注 60min ,Sh、Is、Po和Ip组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别为 0 2 3± 0 0 5、0 73± 0 12 ;0 46± 0 0 4、1 2 3± 0 11;0 2 7± 0 0 4、0 80± 0 12 ;0 2 9± 0 0 3、0 87± 0 0 7(mmol/L)。Is、Po和Ip组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别约为Sh组 2 0 3 %、169% (P <0 0 1) ;118%、110 % (P >0 0 5 ) ;13 1%、119% (P >0 0 5 )。与Is组相比 ,Ip组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别减少约 3 7% (P<0 0 1)和 2 9% (P <0 0 1) ;Po组海马中 2 ,3 -DHBA、2 ,5 -DHBA含量分别减少约 41% (P <0 0 1)和 3 5 % (P <
Objective: To investigate the effect of ischemic preconditioning on the content of hydroxyl free radical in hippocampus after gerbil ischemia-reperfusion in order to explore its possible mechanism of neuroprotection. Methods: The model of gerbil forebrain ischemia-reperfusion was to occlude bilateral common carotid arteries for 5 min, then release and reperfusion. The gerbils were randomly divided into sham operation group (Sh group), ischemia group (Is group), transient ischemic group (Po group) and ischemic preconditioning group (Ip group), with 6 rats in each group. 30 min before the animals were killed by intraperitoneal injection of sodium salicylate 10 0mg / kg. At 60 min after reperfusion, the hippocampus was rapidly decapitated and the hippocampus was separated on ice. The hippocampal 2,3-dihydroxybenzoic acid (2, 3-DHB), 2 , 5 - dihydroxybenzoic acid (2, 5 - DHBA) content (mmol / L). Results: The contents of 2,3-DHBA, 2,5-DHBA in the hippocampus of Sh, Is, Po and Ip groups were 0 2 3 ± 0 0 5,0 73 ± 0 12 at 60 min after cerebral ischemia-reperfusion in gerbils 46 ± 0 0 4,1 2 3 ± 0 11; 0 2 7 ± 0 0 4,0 80 ± 0 12; 0 29 ± 0 0 3,0 87 ± 0 0 7 (mmol / L). The contents of 2,3-DHBA, 2,5-DHBA in the hippocampus of Is, Po and Ip groups were about 203%, 169% (P <0.01), 118% and 110% 5); 13 1%, 119% (P> 0 0 5). Compared with Is group, the levels of 2,3-DHBA, 2,5-DHBA in hippocampus of Ip group decreased by about 37% (P <0.01) and 29% (P <0.01), respectively; The contents of 2,3-DHBA, 2,5-DHBA decreased by 41% (P <0.01) and 35% (P <