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据作者所知,目前还没有使用未经培养的羊水细胞进行遗传病的产前诊断研究。本文研究了41名孕妇(27名中正常一胎、6名无脑儿、5名脊柱裂和3名其它异常),由于血清α-胎蛋白升高或以前分娩过受累婴儿表明有神经管缺陷的高危险率时,在妊娠17-22周经腹部进行羊膜穿刺,采集羊水标本,取0.8～1.0ml羊水离心(700转/分钟)10分钟,未经培养的羊水细胞直接涂片,每张玻片上放置50μl的浓缩细胞悬液。涂片用
To the best of my knowledge, prenatal diagnosis of genetic diseases has not been studied using uncultured amniotic fluid cells. In this paper, 41 pregnant women (27 normal, one without brain, five spina bifida, and three other abnormalities) were studied. Infants with neural tube defects due to elevated serum alpha-fetoprotein or previous delivery Amniocentesis was performed in the abdomen at 17-22 weeks of gestation, amniotic fluid samples were collected, and amniotic fluid samples were collected by centrifugation at 0.8-1.0 ml (700 rev / min) for 10 minutes. The uncultured amniotic fluid cells were directly smeared, each Place 50 μl of the concentrated cell suspension on the slide. Smear use