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目的:通过观察海地瓜体壁的亚显微结构,对它的质地做出合理的解释,选择合理的加工技术;探讨海地瓜的水解产物的体内抗氧化作用。方法:用电镜扫描法观察海地瓜体壁结构;通过正交实验确定复合蛋白酶酶解海地瓜的最适条件。制得海地瓜水解液,灌胃大鼠。56d后,测定大鼠血清和肝脏组织匀浆液中SOD,GSH-Px活力。结果与结论:电镜扫描分析发现海地瓜的肌纤维丝较粗壮,排列凌乱,纤维丝排列紧密。海地瓜肌肉组织的这种排列方式可能是它的肌肉坚韧,难于咀嚼的原因。正交实验确定海地瓜的最佳酶解条件为:2%复合蛋白酶,10%底物,酶解温度55℃,酶解时间70min。水解液稀释一倍,灌胃大鼠。发现灌胃海地瓜水解液的大鼠血液中SOD和GSH-Px的活力和肝脏组织中SOD活力比较空白组具有显著提高,说明海地瓜水解液具有良好的抗氧化活性。
OBJECTIVE: To investigate the sub-microstructure of the body wall of Haitian melon and to make a reasonable explanation of its texture, and to choose a reasonable processing technology. To explore the antioxidant effect of the hydrolyzate of Haitian melon in vivo. Methods: Electron microscopy was used to observe the wall structure of Haitian melon. The optimal conditions for enzymolysis of Haitian melon by compound protease were determined by orthogonal experiment. Prepared Haitian melon hydrolyzate, stomach rats. After 56 days, the activities of SOD and GSH-Px in serum and liver homogenate of rats were determined. RESULTS AND CONCLUSION: Scanning electron microscopy showed that the mycelial filaments of Haitian melon were thicker and arranged in a disorderly manner, and the filaments arranged closely. This arrangement of sea cucumber muscle tissue may be its muscle tough, difficult to chew reasons. Orthogonal experiments to determine the optimum conditions for the hydrolysis of sweet potatoes: 2% compound protease, 10% substrate, enzymatic temperature 55 ℃, hydrolysis time 70min. Dilute the hydrolyzate, stomach rats. It was found that the activity of SOD and GSH-Px in the blood of rats fed with hydrolyzate of Haitian melon and the activity of SOD in liver tissue were significantly increased, indicating that Haitian melon hydrolyzate has good anti-oxidative activity.