论文部分内容阅读
目的调查河南地区维生素D受体(VDR)基因SNP位点rs2222570和结核病易感性之间关系。方法研究采用以医院为基础的病例对照研究方法,病例组纳入260个结核菌菌阳患者,并以年龄和性别相匹配纳入258名健康体检人群作为对照组。采用聚合酶链式反应-限制性片段长度多态性对VDR基因SNP位点rs2222570多态性进行分析。结果在本次研究中258例对照人群中VDR基因C/C,C/T和T/T三种基因型分别占110(42.6%),106(41.1%),42(16.3%);在260例结核病患者中VDR基因C/C,C/T and T/T三种基因型分别占70(26.9%),130(50%),60(23.1%),二者分布存在统计学差异(=14.499,P=0.001)。单因素分析表明VDR基因SNP位点rs2222570多态性与结核病患病风险相关,VDR基因野生型C/C与杂合型C/T和突变型T/T相比降低了结核病发病50%的风险(校正OR=0.496,95%CI:0.344-0.717)。结论在河南地区VDR受体基因多态性与结核病发病相关。
Objective To investigate the relationship between the SNP loci rs2222570 of vitamin D receptor (VDR) gene and susceptibility to tuberculosis in Henan Province. METHODS: A hospital-based case-control study was conducted. The case group included 260 patients with tuberculosis bacilli and included 258 healthy people as control group by age and sex. Polymerase chain reaction-restriction fragment length polymorphism was used to analyze rs2222570 SNP locus in VDR gene. Results The CDR, C / C, C / T and T / T genotypes accounted for 110 (42.6%), 106 (41.1%) and 42 (16.3%) in 258 control subjects in this study. Cases of tuberculosis patients with VDR gene C / C, C / T and T / T three genotypes accounted for 70 (26.9%), 130 (50%), 60 (23.1%), the distribution of the two statistically significant difference 14.499, P = 0.001). Univariate analysis showed that rs2222570 polymorphism of VDR gene SNP locus was associated with the risk of tuberculosis. VDR gene wild type C / C reduced the risk of tuberculosis by 50% compared with heterozygous C / T and mutant T / T (Corrected OR = 0.496, 95% CI: 0.344-0.717). Conclusion The polymorphism of VDR receptor gene in Henan is related to the pathogenesis of tuberculosis.