An eccentric core single-mode fiber, whose core is away from the axis of the fiber, is fabricated by using the fiber preform goniometric-groove method and the stack-and-draw method.An eccentric core single-mode fiber without coating is spliced between two single-mode fibers.Fundamental mode and cladding modes are excited at one splicing point between the eccentric core single-mode fiber and the single-mode fiber, and propagate in the eccentric core single-mode fiber, then interfere with each other at the other splicing point.Multi-beam interference transmission spectrum is observed.An in-line fiber interferometric strain sensor based on the eccentric core single-mode fiber is realized.