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目的 :制备EDA蛋白的多克隆抗体。方法 :用含EDA蛋白的蛋白凝胶免疫兔子 ,用ELISA和West ernblotting方法检测抗血清效价。结果 :两只动物中一只产生了高效价的抗血清 ,另一只产生的效价较低。结论 :用含EDA蛋白的蛋白凝胶免疫兔子可获得了高效价的抗血清 ,为深入研究该蛋白在牙齿发育中的具体作用奠定了基础
Objective: To prepare polyclonal antibody to EDA protein. Methods: Rabbits were immunized with protein gel containing EDA protein, and antisera titers were detected by ELISA and West ernblotting method. Results: One of the two animals produced a high titer antiserum while the other produced a lower titer. CONCLUSION: High titer anti-serum is obtained by immunizing rabbits with protein gel containing EDA protein, which lays the foundation for further study on the specific role of this protein in tooth development