目的:建立一种曙红Y为探针的选择好、快速、简便测定盐酸丁卡因(TA·HCl)的新方法。方法:在pH 3.3的Britton-Robinson(BR)缓冲溶液中,于513 nm处测定溶液的吸光度。结果:该法的摩尔吸光系数(ε)为1.94×10~4L·~(-1),线性范围为0.3～4.2μg·ml~(-1)(r=0.999 8)。结论:该方法可用于人血清及尿样中盐酸丁卡的含量测定。 Objective: To establish a new method for the determination of tetracaine hydrochloride (TA · HCl) using Eosin Y as a probe. Methods: The absorbance of the solution was measured at 513 nm in a Britton-Robinson (BR) buffer solution at pH 3.3. Results: The molar absorption coefficient (ε) of this method was 1.94 × 10 ~ 4L ··L -1 with a linear range of 0.3 ~ 4.2μg · ml -1 (r = 0.999 8). Conclusion: This method can be used to determine the content of dinkatlon hydrochloride in human serum and urine samples.