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目的对四川主产黄连进行了测试性状的选择与初步分析,为制订黄连特异性、一致性和稳定性(DUS)测试指南提供科学依据。方法选择黄连的48个形态性状,包括12个必测性状和36个补充性状,分析数量性状的差异性;探讨HPLC指纹图谱在黄连DUS测试应用,分析了不同品种的黄连差异性,作为鉴定辅助手段。结果黄连的数量性状受环境与生长年限影响大,许多性状均有较大变异。采用HPLC-FPS曲线图分析不同品种的黄连,差异性都比较明显。结论制订测试指南时应以植物形态特征为主,品质性状和生理生化性状为辅。如果形态性状难以区分,可将化学成分指纹图谱技术作为辅助鉴定手段。
OBJECTIVE: To provide a scientific basis for the development of the test of specificity, consistency and stability (DUS) of Coptis chinensis. Methods Forty-eight morphological traits of Coptis chinensis, including 12 mandatory traits and 36 supplementary traits, were selected to analyze the differences in quantitative traits. The HPLC fingerprinting was applied to the DUS test of Coptis chinensis to analyze the difference of Coptis chinensis means. Results The quantitative characters of Coptis chinensis were greatly influenced by the environment and growth years, and many traits showed great variation. The HPLC-FPS curve analysis of different varieties of Coptis, the differences are more obvious. Conclusion The test guidelines should be based on plant morphology, supplemented by quality traits and physiological and biochemical traits. If morphological traits are difficult to distinguish, chemical fingerprinting can be used as a secondary method of identification.