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本研究应用人两倍体纤维母细胞(SL_7),直接从中国不同地区来源的甲肝病人粪便中分离到三株甲肝病毒,初次甲型抗原出现时间较长,潜伏期分别从50天至125天不等,但经连续传代后,潜伏期可缩短至9～10天。研究中主要采用免疫荧光法监测、随访细胞中甲肝抗原的出现情况,故能较早发现阳性结果。进一步研究发现,甲肝抗原均分布于感染的细胞浆内,呈细沙粒样分布,阳性细胞根据感染程度可从局灶性扩散至几乎全部的细胞层,但与感染的PLC/PRF/5肝癌细胞的甲肝抗原表现不同,无粗大的荧光染色块出
In this study, human diploid fibroblasts (SL_7) were used to isolate three Hepatitis A virus directly from the excrement of hepatitis A patients from different regions of China. The initial appearance of type A antigen was longer, and the incubation period was from 50 days to 125 days However, after continuous passage, the incubation period can be shortened to 9 to 10 days. The study mainly used immunofluorescence monitoring, follow-up of cells in the presence of hepatitis A antigen, it can be found earlier positive results. Further study found that hepatitis A antigens are distributed in the infected cytoplasm, showed a fine sand-like distribution, according to the degree of infection of positive cells can spread from focal to almost all of the cell layer, but with infected PLC / PRF / 5 liver cancer Hepatitis A antigen cells show different performance, no gross fluorescent staining out