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目的探讨本地区临床分离多重耐药大肠埃希菌(Escherichia coli,E.coli)的氨基糖苷类修饰酶和I类整合子基因存在情况。方法纸片扩散法(K-B法)测定71株大肠埃希菌对常用抗菌药物的耐药性,应用PCR技术对E.coli进行氨基糖苷类修饰酶和I类整合酶基因的检测。结果 71株大肠埃希菌对庆大霉素、左氧氟沙星、头孢噻肟、头孢他啶的耐药率分别分为66.20%、63.38%、59.15%、18.31%,其中23株为多重耐药株(32.39%)。氨基糖苷类修饰酶基因aac(3)-II阳性40株(56.34%),aac(6’)-I阳性22株(30.99%),ant(3“)-I阳性14株(19.72%),未检出aac(6’)-II阳性株,氨基糖苷类修饰酶基因总检出率为74.65%;I类整合酶基因(intI1)阳性53株(74.65%)。多重耐药与非多重耐药大肠埃希菌中aac(3)-II、aac(6’)-I、总氨基糖苷类修饰酶基因、intI1的阳性率有统计学差异(P<0.05)。结论本地区多重耐药E.coli的氨基糖苷类修饰酶基因、I类整合酶基因携带率高。
Objective To investigate the presence of aminoglycoside-modifying enzymes and class I integron genes in clinical isolates of Escherichia coli (E.coli) in the region. Methods The resistance of 71 Escherichia coli strains to commonly used antibiotics was determined by disk diffusion method (K-B method). The aminoglycoside-modifying enzyme and the integrase gene of E.coli were detected by PCR. Results The resistance rates of 71 strains of Escherichia coli to gentamicin, levofloxacin, cefotaxime and ceftazidime were respectively 66.20%, 63.38%, 59.15% and 18.31%, of which 23 were multidrug resistant strains (32.39% ). 40 (56.34%) positive for aAG (3) -II gene, 22 (30.99%) positive for aac (6 ’) - I and 14 (19.72%) positive for ant (3 ”) , And the positive rate of aminoglycoside modified enzyme was 74.65%, while the positive rate of intI1 was 53 (74.65%). The multi-resistant and non-multiple The positive rates of aac (3) -II, aac (6 ’) - I, total aminoglycoside modifying enzyme gene and intI1 in drug - resistant Escherichia coli were statistically different (P <0.05) The aminoglycoside-modifying enzyme gene of E. coli and the class I integrase gene carry rate is high.