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目的研究天麻素(Gastrodin)对肿瘤、非肿瘤2种不同生理病理状态下化疗诱导的神经病理性疼痛(化疗痛,Chemotherapy-induced Neuropathic Pain,CINP)大鼠模型的作用。方法采用腹腔注射长春新碱(Vincristine,VCR,125μg·kg~(-1))建立正常大鼠和Walker-256乳腺癌荷瘤大鼠CINP动物模型,并腹腔注射给予不同剂量的天麻素(60,120 mg·kg~(-1));采用Von Frey电子测痛仪检测大鼠机械刺激痛阈值,PL-200型热刺痛仪测定大鼠热刺激痛阈值。结果 VCR对肿瘤、非肿瘤大鼠均可诱导产生机械痛敏反应和热痛敏反应,使其痛阈值均明显降低(P<0.01);天麻素可剂量依赖性抑制肿瘤、非肿瘤CINP大鼠模型的机械痛敏反应和热痛敏反应(P<0.01)。将大鼠注射VCR前后的痛阈值差值以及天麻素治疗前后大鼠痛阈值差值进一步比较,VCR对非肿瘤组大鼠机械痛痛阈值的降低幅度比肿瘤组的明显(P<0.01),而VCR对肿瘤组的热痛阈值降低幅度比非肿瘤组明显,但差异无统计学意义(P>0.05);天麻素对非肿瘤组大鼠机械痛阈值的提高作用比肿瘤组明显(P<0.05),而天麻素对肿瘤组大鼠的热痛阈值提高作用比非肿瘤组明显(P<0.05)。结论天麻素对VCR诱导的肿瘤、非肿瘤大鼠CINP均有剂量依赖性抑制作用;但对生理、病理状态下大鼠的不同感觉神经纤维的痛觉抑制作用敏感性存在差异,对VCR诱导痛敏反应越敏感的神经纤维其抑制作用也相对越强。
Objective To investigate the effect of Gastrodin on the chemotherapy-induced Neuropathic Pain (CINP) rat model induced by chemotherapy and two different physiological and pathological conditions. Methods CINP animal models of normal rats and Walker-256 breast cancer bearing mice were established by intraperitoneal injection of Vincristine (VCR, 125 μg · kg -1) and intraperitoneally injected with different doses of gastrodin (60, 120 mg · kg ~ (-1)). Von Frey electronic analgesia was used to detect the mechanical stimulation threshold of pain in rats. PL-200 thermal tachycardia was used to measure the thermal stimulation threshold. Results VCR could induce both mechanical hyperalgesia and hyperalgesia in both tumor and non-tumor rats, and their pain thresholds were significantly decreased (P <0.01). Gastrodin could inhibit tumor and non-tumor CINP rats in a dose-dependent manner Mechanical allodynia and thermal hyperalgesia (P <0.01). Compared with the difference of pain threshold before and after injection of VCR in rats and the difference of pain threshold between before and after gastrodin treatment, the reduction of mechanical pain threshold of VCR in non-tumor group was significantly lower than that in tumor group (P <0.01) However, the decrease threshold of thermal pain in VCR group was higher than that in non-tumor group, but the difference was not statistically significant (P> 0.05). Gastrodin increased the mechanical pain threshold in non-tumor group significantly (P < 0.05), while the effect of gastrodin on the heat pain threshold in the tumor group was significantly higher than that in the non-tumor group (P <0.05). Conclusion Gastrodin has a dose-dependent inhibitory effect on VCR-induced tumor and non-tumor-bearing rat CINP. However, the sensitivity of sensory nerve fibers to different sensory nerve fibers in physiological and pathological conditions is different. Volatile sensitivity The more sensitive the reaction of nerve fibers its inhibitory effect is relatively stronger.