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目的 建立单侧缺氧缺血性脑损伤 (HIBD)动物模型 ,研究胰岛素样生长因子 1(IGF 1)对HIBD的影响和可能机制。 方法 选择健康 7日龄Wistar大鼠 12 0只 ,建立HIBD模型 ,随机分成假手术组、HIBD组、HIBD后 0 .2mg/kg人基因重组IGF 1干预组 (RH IGF 1组 )、0 .0 6 6mg/kg人基因重组IGF 1干预组 (SRH IGF 1组 )及盐水对照组 (对照组 )。各组按观察时段进一步分为 2 4、4 8、72h组 ,每组 8只。各组于规定时刻观测脑形态学改变、谷氨酸 (Glu)含量、凋亡细胞计数、Bcl 2蛋白表达。 结果 (1)HIBD 4 8h组Glu(116 2 .2± 10 8.1)mg/kg ,较假手术组(75 0 .9± 5 3.4 )mg/kg明显升高 (P <0 .0 5 ) ;HIBD组凋亡细胞计数 [2 4h :(7.6± 1.9) % ,4 8h(12 .6±1.2 ) % ,72h :(13.8± 0 .9) % ],较假手术组 [2 4h(2 .0± 0 .2 ) % ,4 8h(2 .0± 0 .3) % ,72h(2 .0±0 .2 ) % ]明显增加 (P均 <0 .0 5 )。 (2 )与对照组相比 ,RH IGF 1组脑组织病变减轻 ;干预 4 8h组Glu[SRH IGF 1组 (781.4± 5 4 .2 )mg/kg ,RH IGF 1组 (74 0 .5± 4 6 .6 )mg/kg],较对照组 (112 6 .6± 4 8.0 )mg/kg明显降低 (P均 <0 .0 5 ) ;RH IGF 1组凋亡细胞计数 [2 4h :(3.6± 0 .9) % ,4 8h(8.2± 2 .2 ) % ,72h(9.4± 1.4 ) % ],较对
Objective To establish an animal model of unilateral hypoxic-ischemic brain damage (HIBD) and investigate the effect of insulin-like growth factor 1 (IGF-1) on HIBD and its possible mechanism. Methods A total of 120 healthy 7-day-old Wistar rats were selected to establish HIBD model and were randomly divided into sham operation group, HIBD group, 0.2 mg / kg human recombinant IGF 1 group (RH IGF 1 group) 6 6mg / kg human recombinant IGF 1 intervention group (SRH IGF 1 group) and saline control group (control group). Each group according to the observation period is further divided into 2 448,72h group, 8 in each group. The rats in each group were observed morphological changes, glutamic acid (Glu) content, apoptotic cell counts and Bcl 2 protein expression at the prescribed time points. Results (1) Glu (116.2 ± 10 8.1) mg / kg in HIBD 4 8h group was significantly higher than that in sham operation group (75.09 ± 5.44) mg / kg (P <0.05). The number of apoptotic cells in HIBD group was significantly higher than that in sham operation group [24 h: (7.6 ± 1.9)%, 48 h (12.6 ± 1.2)%, 72 h: (13.8 ± 0.9)%] 0 ± 0.2%), 48h (2.0 ± 0.3%), 72h (2.0 ± 0.2%)] significantly increased (all P <0.05). (2) Compared with the control group, the brain lesions in RH IGF1 group were alleviated; the levels of Glu [SRH IGF1 group (781.4 ± 54.2) mg / kg and RH IGF1 group 4 6 .6 mg / kg], which was significantly lower than that of the control group (112 6 .6 ± 4 8.0) mg / kg (P all <0.05) 3.6 ± 0.9)%, 48h (8.2 ± 2.2)%, 72h (9.4 ± 1.4)%]