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目的:了解黔东南州2010年手足口病流行的主要病原体。方法:采集临床诊断为手足口病病例的咽拭子、粪便或肛拭子、疱疹液采用RT-PCR方法扩增病毒的特异性片段,检测EV71、CA16核酸和非EV71、CA16的其他肠道病毒核酸。结果:2010年4月-2010年11月共检测标本101份,病毒核酸检测阳性50份(49.50%),其中EV71为17.82%;CA16为19.80%;其他肠道病毒阳性为11.88%。101例标本中,从发病至采样0 d~2 d病毒核酸检出率最高(59.68%),其次是3 d~5 d(39.29%)。咽拭子、肛拭子或大便、疱疹液阳性率分别为48.42%、50.00%和100%。结论:黔东南州2010年手足口病流行毒株以肠道病毒CA16和EV71为主。
Objective: To understand the main pathogens of the epidemic of hand, foot and mouth disease in Qiandongnan in 2010. Methods: Throat swabs, stools or anal swabs were collected for the diagnosis of hand-foot-mouth disease. Herpes simplex virus-specific fragments were amplified by RT-PCR. The EV71 and CA16 nucleic acids and other intestinal Viral nucleic acid. Results: From April 2010 to November 2010, 101 samples were detected and 50 (49.50%) were positive for virus nucleic acid, of which EV71 was 17.82%, CA16 was 19.80%, and other enterovirus positive was 11.88%. Of the 101 samples, the highest detection rate of nucleic acid (59.68%) was found from 0 days to 2 days after sampling, followed by 3 days to 5 days (39.29%). The positive rates of throat swab, anal swab or stool and herpes fluid were 48.42%, 50.00% and 100% respectively. Conclusion: In 2010, the epidemic strains of HFMD in Qiandongnan Prefecture were mainly enterovirus CA16 and EV71.