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以不同浓度的苯并 (a)芘代谢物反式二羟环氧苯并芘 (BPDE)多次处理人支气管上皮细胞 16HBE ,并观察转化细胞的恶性特征。发现BPDE可诱导 16HBE细胞恶性转化 ,形成转化灶。转化灶细胞失去接触抑制 ,排列紊乱 ,无方向性 ,交叉重叠生长。转化的细胞可在软琼脂上生长 ,各浓度处理组细胞集落形成率均显著高于对照组 ,有良好的剂量—反应关系。经BPDE处理的细胞在裸鼠体内成瘤 ,病理学诊断为鳞状细胞癌。本实验以反式BPDE成功地诱发了人支气管上皮细胞恶性转化 ,为后期进一步研究其致癌的分子机制、寻找致癌相关基因提供了理想的生物学材料。
Human bronchial epithelial cells 16HBE were treated several times with different concentrations of the benzo(a) pyrene metabolite trans-dihydroxyepoxybenzo (BPDE), and the malignant characteristics of transformed cells were observed. BPDE was found to induce malignant transformation of 16HBE cells and form foci. Transformant cells lost contact inhibition, disordered arrangement, non-directional, overlapping growth. The transformed cells can grow on soft agar. The colony formation rate of the treated groups at each concentration was significantly higher than that of the control group, and there was a good dose-response relationship. The BPDE-treated cells were tumorigenic in nude mice and the pathological diagnosis was squamous cell carcinoma. In this experiment, the malignant transformation of human bronchial epithelial cells was successfully induced by trans BPDE, providing an ideal biological material for the further study of its molecular mechanism of carcinogenesis and search for carcinogenic related genes.