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采用紫外-可见光谱法(UV-Vis)研究三种二茂铁衍生物[Fc(COOH)_2(λ_(max)=286nm),Fc(OBt)_2(λ_(max)=305nm),Fc(Cys)(λ_(max)=289nm)]与血红素(heme,λ_(max)=386nm)的相互作用。实验结果表明:当固定heme浓度时,heme的吸光度随着Fc(COOH)_2和Fc(Cys)浓度的增加而增大,而heme的吸光度随着Fc(OBt)_2的浓度的增加几乎没有增大;当分别固定Fc(COOH)_2,Fc(Cys)和Fc(OBt)_2的浓度时,Fc(COOH)_2和Fc(Cys)的吸光度随着heme浓度的增加而增大,而Fc(OBt)_2的吸光度随着heme浓度的增加没有变化,说明Fc(COOH)_2和Fc(Cys)与heme存在分子间的相互作用,主要是由于Fc(COOH)_2和Fc(Cys)与heme能形成氢键,分子链增长,吸收的能量增加,导致吸光度增大;而Fc(OBt)_2与heme没有分子间的相互作用,是由于Fc(OBt)_2没有自由的氢,不能与heme形成分子间的氢键。同时考察了三种二茂铁衍生物与heme的吸光度随时间的变化,Fc(COOH)_2和Fc(Cys)与heme的吸光度随着时间的增加而减少,而Fc(OBt)_2与heme的吸光度随时间的变化几乎没有变化。Fc(COOH)_2与Fc(Cys)和heme的反应时间为0.5,18和48h,当固定Fc(COOH)_2浓度时,在λ_(max)=384nm处的吸光度由2.64分别变为2.53和2.51;当固定heme的浓度时,在λ_(max)=384nm处的吸光度由1.76分别变为1.72和1.68;当固定Fc(Cys)浓度时,在λ_(max)=397nm处的吸光度由2.74分别变为2.63和2.55;当固定heme的浓度时,在λ_(max)=397nm处的吸光度由1.82分别变为1.58和1.49。
Three kinds of ferrocene derivatives [Fc (COOH) _2 (λ max = 286 nm), Fc (OBt) _2 (λ max = 305 nm) and Fc Cys) (λ max = 289 nm)] with heme (λ max = 386 nm). The experimental results showed that when heme concentration was fixed, the absorbance of heme increased with the increasing of Fc (COOH) 2 and Fc (Cys), while the absorbance of heme increased little with the increase of Fc (OBt) 2 The absorbance of Fc (COOH) 2 and Fc (Cys) increased with the increase of heme concentration when the concentrations of Fc (COOH) 2, Fc (Cys) and Fc (OBt) The results showed that the interaction between Fc (COOH) _2 and Fc (Cys) and heme existed mainly due to the interaction between Fc (COOH) _2 and Fc (Cys) The formation of hydrogen bonds, the molecular chain increases, the absorption of energy increases, resulting in increased absorbance; and Fc (OBt) _2 and heme no intermolecular interaction is due to Fc (OBt) _2 free hydrogen can not form a molecule with heme Between the hydrogen bonds. The changes of absorbance of three ferrocene derivatives and heme with time were also investigated. The absorbance of Fc (COOH) _2 and Fc (Cys) and heme decreased with the increase of time, while Fc (OBt) _2 and heme Absorbance changes little over time. The reaction time of Fc (COOH) _2 with Fc (Cys) and heme was 0.5, 18 and 48 h, respectively. When the concentration of Fc (COOH) _2 was fixed, the absorbance at λ max = 384 nm changed from 2.64 to 2.53 and 2.51 ; When the heme concentration was fixed, the absorbance at λ max = 384 nm changed from 1.76 to 1.72 and 1.68, respectively; when the Fc (Cys) concentration was fixed, the absorbance at λ max = 397 nm was changed from 2.74 Was 2.63 and 2.55, respectively; when the heme concentration was fixed, the absorbance at λ max (max) = 397 nm changed from 1.82 to 1.58 and 1.49, respectively.