目的　探讨膀胱移行细胞癌中热休克蛋白 2 7(HSP2 7)和 DNA拓扑异构酶 α(To Po α)的表达。方法　 SP免疫组织化学染色 ,检测 85例膀胱癌患者病灶组织中 HSP2 7和 To Po α的表达率。结果　 HSP2 7阳性率为 4 7.0 6 % (4 0 / 85 ) ,To Po α阳性率为 5 8.82 % (5 0 / 85 ) ,两项同时阳性者占 35 .2 9% (30 / 85 )。To Po α表达与移行细胞癌病理的分级呈正相关 , 级明显低于 和 级 (P<0 .0 1) ;HSP2 7表达 级中高于 、 级 ,但无显著性差异 (P>0 .0 5 )。结论　检测膀胱移行细胞癌中 HSP2 7和 To Po α可以指导化疗方案的制定。 Objective To investigate the expression of heat shock protein 27 (HSP2 7) and topo α in bladder transitional cell carcinoma. Methods SP immunohistochemical staining was used to detect the expression of HSP27 and To Po α in 85 cases of bladder cancer. Results The positive rate of HSP27 was 4 7.06% (40/85), the positive rate of To Po a was 58.82% (50/85), and the two positives were 35.29% (30/85). The expression of To Po α was positively correlated with the pathological grade of transitional cell carcinoma, and the level of To Po α was significantly lower than that of grade (P <0.01). The expression level of HSP 2 7 was higher than that of grade 2, but there was no significant difference (P> 0.05) ). Conclusion Detecting bladder transitional cell carcinoma of HSP27 and To Po α can guide the development of chemotherapy regimen.