论文部分内容阅读
目的 :研究成人咽喉部良、恶性病变与人乳头状瘤病毒 (HPV)感染的关系。方法 :应用聚合酶链反应 (PCR)和斑点杂交技术 ,对 5 5例咽喉不同病变的新鲜组织标本进行 HPV6,1 1 ,1 6,1 8,33共 5型HPV- DNA感染的检测。结果 :在咽乳头状瘤组 HPV感染率为 6 0 % (6 / 1 0 ) ,喉乳头状瘤组为 70 % (7/1 0 ) ,喉鳞状上皮非典型增生组为 2 0 % (1 / 5 ) ,声带息肉组为 2 0 % (1 / 5 ) ,喉癌组为 2 0 % (1 / 5 ) ,声带小结组为 0 (0 / 1 0 )。HPV- DNA型别分布在咽喉良性病变中以 HPV6,1 1 型为主 ,喉癌中以 HPV1 6为主。结论 :本地区成人咽喉良、恶性病变的发生与 HPV感染密切相关。PCR结合核酸斑点杂交法检测 HPV,具有灵敏性高、特异性强的优点 ,值得推广应用。
Objective: To study the relationship between benign and malignant lesions of throat and human papillomavirus (HPV) infection in adults. Methods: Polymerase chain reaction (PCR) and dot blot hybridization were used to detect HPV-1, HPV-1, HPV-1 and HPV-DNA in 5 specimens of different pathological changes of throat. Results: The HPV infection rate was 60% (6/10) in the pharyngological papilloma group, 70% (7/1) in the laryngeal papilloma group and 20% in the laryngeal squamous cell atypical hyperplasia group 1/5), 20% (1/5) in vocal cord polyps, 20% (1/5) in laryngocarcinoma, and 0 (0/10) in vocal nodules. HPV-DNA type distribution in the benign lesions of the throat to HPV6, type 1 1, HPV16 mainly in laryngeal cancer. Conclusion: The incidence of benign and malignant throat in adults in this area is closely related to HPV infection. PCR combined with nucleic acid dot blot hybridization detection of HPV, with high sensitivity and specificity of the merits, it is worth promoting.