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利用红外光谱的宏观指纹特性,对短瓣金莲花不同溶剂提取部位的红外光谱图和二阶导数红外光谱图进行了整体分析。综合分析可知,短瓣金莲花石油醚提取部位主含长链烷烃、脂肪酸或酯类成分,乙酸乙酯提取部位主含长链脂肪酸(酯)及黄酮类化合物,95%乙醇提取部位主含黄酮及黄酮苷类化合物,60%乙醇提取部位主含黄酮苷及多糖类成分,30%乙醇和去离子水提取部位主含多糖类成分。因此,利用红外光谱法可从整体上对中药不同提取部位的主体成分进行直接、快速的分析,为中药提取物的工艺优化及其分析研究提供有价值的参考和方向性指导。
By using the macroscopic fingerprints of infrared spectroscopy, the infrared spectra and the second derivative infrared spectra of different solvent parts were analyzed. Comprehensive analysis shows that the main part of the extracted fraction of the petals of the genus Petal contains long-chain alkanes, fatty acids or esters. The ethyl acetate fraction mainly contains long-chain fatty acid esters and flavonoids, and the main part of the 95% ethanol extract contains flavonoids And flavonoid glycosides, flavonoid glycosides and polysaccharides are mainly contained in the 60% ethanol extracting parts, polysaccharide components are mainly contained in the extracting parts of 30% ethanol and deionized water. Therefore, using infrared spectroscopy can directly and rapidly analyze the main components of different parts of traditional Chinese medicine as a whole, and provide valuable reference and directional guidance for the process optimization and analysis of traditional Chinese medicine extracts.