论文部分内容阅读
霍乱的病原菌是Ｏ１群霍乱弧菌，但在Ｏ１群霍乱弧菌中，仅有部分可以引起霍乱流行，因此不同菌株在流行病学上的意义差异很大。为探讨Ｏ１群菌不同型别和亚群之间的遗传关系和进化变异规律，应用多位点酶电泳法对Ｏ１群霍乱弧菌进行研究。结果：Ｏ１群霍乱弧菌的大多基因位点等位基因频率较低，尤其是古典型菌株和埃尔托型流行株，大多被检测基因为非多态位点；Ｏ１群霍乱弧菌的平均遗传多态值（ＨＯ１）为０．１８３，平均杂合度（ｈＯ１）为５．６７；其中古典型菌、埃尔托型流行株、埃尔托型非流行株的平均遗传多态值和平均杂合度分别为：ＨＣＶＣ＝０．１０６，ｈＣＶＣ＝１．５；ＨＥＳＥＶＣ＝０．０５６，ｈＥＳＥＶＣ＝２．３；ＨＮＳＥＶＣ＝０．４２５，ｈＮＳＥＶＣ＝５。结果显示：古典型和埃尔托型流行株作为霍乱的病原体是一个遗传关系紧密的群体，而埃尔托型非流行株遗传关系较为离散，与流行株之间的关系也较远，可能是其长期作为环境菌株进化变异的结果
The pathogen of cholera is Vibrio cholerae O1 group, but only part of the V. cholerae O1 group can cause cholera epidemic, so the epidemiological significance of different strains vary greatly. To investigate the genetic relationship and evolutionary variation between different types and subgroups of O1 group bacteria, multi-site enzyme electrophoresis was used to study the Vibrio cholerae O1 group. Results: The frequency of most alleles of Vibrio cholerae O1 was low, especially for classical strains and Elto type strains. Most of the tested genes were non-polymorphic. The average of Vibrio cholerae O1 The average value of genetic polymorphism (HO1) was 0.183, and the average heterozygosity (hO1) was 5.67. The average genetic polymorphism value of the classical, Elto and Elto non-epidemic strains was Heterozygosity were: HCVC = 0.106, hCVC = 1.5; HESEVC = 0.056, hESEVC = 2.3; HNSEVC = 0.425, hNSEVC = 5. The results showed that classical and Elto type strains were a closely related group as pathogen of cholera. However, Elto type non-epidemic strains were more discrete in genetic relationship and distantly related to epidemic strains, probably Its long-term as a result of evolutionary variation of environmental strains