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目的研究p,p’-DDE和β-BHC联合染毒对大鼠离体支持细胞脂质过氧化的影响。方法通过离体培养SD大鼠支持细胞的四甲基偶氮唑盐(MTT)实验,确定后续实验的染毒剂量,p,p’-DDE和β-BHC的染毒浓度均为10、30、50μmol/L,p,p’-DDE+β-BHC联合染毒浓度为10μmol/Lp,p’-DDE+10μmol/Lβ-BHC,30μmol/Lp,p’-DDE+30μmol/Lβ-BHC,50μmol/Lp,p’-DDE+50μmol/Lβ-BHC,检测染毒后支持细胞乳酸脱氢酶(LDH)的漏出率、总超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的变化。结果50μmol/L的p,p’-DDE、β-BHC均可使支持细胞的吸光度(A)值显著下降(P<0.05)。睾丸支持细胞LDH的漏出率随染毒浓度的增加而明显增加(P<0.05),二者以不同浓度联合染毒时支持细胞LDH的漏出率均显著高于对应浓度的单独染毒(P<0.05)。不同浓度的p,p’-DDE、β-BHC及二者联合染毒均可使细胞内总SOD活力显著降低(P<0.05)、MDA的含量增加(P<0.05),且联合染毒时MDA的含量与二者单独染毒时相比均有显著增加(P<0.05)。结论p,p’-DDE、β-BHC单独和联合作用均可以引发睾丸支持细胞脂质过氧化,p,p’-DDE和β-BHC对支持细胞的联合毒性作用具有一定的协同效应。
Objective To study the effects of p, p’-DDE and β-BHC on lipid peroxidation in isolated rat ex vivo cells. Methods The dose of p, p’-DDE and β-BHC were determined by in vitro MTT assay. The concentrations of p, p’-DDE and β-BHC were all 10,30 , 50μmol / L, 10μmol / Lp, p’-DDE + 10μmol / Lβ-BHC, 30μmol / Lp, p’-DDE and 30μmol / The leakage rates of lactate dehydrogenase (LDH), the activity of total superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the supportive cells after exposure to 50μmol / Lp, p’-DDE and 50μmol / The change. Results The absorbency (A) of supporting cells was decreased significantly (P <0.05) with 50μmol / L p, p’-DDE and β-BHC. Leakage rate of LDH in testicular support cells increased significantly with the increase of exposure concentration (P <0.05). The leakage rates of LDH in supportive cells were significantly higher than those exposed to corresponding concentration alone (P < 0.05). The combination of p, p’-DDE and β-BHC with different concentrations could significantly decrease the total intracellular SOD activity (P <0.05) and MDA content (P <0.05) The content of MDA increased significantly compared with the control group (P <0.05). Conclusions Both p, p’-DDE and β-BHC can induce lipid peroxidation in testicular support cells alone and in combination. The synergistic effect of p, p’-DDE and β-BHC on the joint toxicity of supportive cells was observed.