对甜橙(Citrus sinensis(L.)Osbeck)成年树的侧芽离体培养进行了研究。试验采用综合指标A值,兼顾存活率、增殖率和质量3个因素,筛选出适宜甜橙侧芽增殖的激素配比;离体新梢茎段的继代增殖须降低激素的浓度。不同甜橙品种对培养基反应不同,存活率、增殖率、质量级别和A值均有显著差异。二步生根法可获得满意生根率。 Lateral bud culture of adult tree of Citrus sinensis (L.) Osbeck was studied in vitro. The experiment adopted comprehensive index A value, taking into account the three factors of survival rate, proliferation rate and quality, and screened hormone proportion suitable for the proliferation of lateral buds of sweet orange. The subculture of stem segments of in vitro shoots should reduce the hormone concentration. Different sweet orange cultivars reacted differently to each other, with significant differences in survival rate, proliferation rate, quality grade and A value. Two-step rooting method can be satisfied with the rooting rate.