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本研究应用放射免疫技术定量测定了小鼠脑、脂肪、肝、肌肉组织及血浆肥胖蛋白[OP-(57-92)]的含量。结果显示:小鼠脑组织OP含量最高,为13.14±0.8pg/mg;脂肪组织中OP含量为2.99±0.54pg/mgl肝脏及肌肉组织中则未检测出OP。雌性小鼠脂肪组织中OP含量明显低于雄性(1.97±0.28vs4.02±0.92pg/mg)。小鼠的血浆中OP含量为2257.8±171.9pg/ml。
In this study, radioimmunoassay was used to quantitatively determine the content of brain fat, liver, muscle tissue and plasma obesity protein [OP- (57-92)] in mice. The results showed that the content of OP in brain tissue of mice was the highest (13.14 ± 0.8pg / mg), the content of OP in adipose tissue was 2.99 ± 0.54pg / mgl, while no OP was detected in liver and muscle. The OP content in adipose tissue of female mice was significantly lower than that of male mice (1.97 ± 0.28 vs 4.02 ± 0.92pg / mg). The mouse plasma OP content was 2257.8 ± 171.9 pg / ml.