使用内部质量控制的酶水解、液-液萃取法提取,GC-ECD及GC-MS/MS检测法对体内组织、体液的三唑仑及α-羟基三唑仑进行检测,能够同时定量检测出其他方法难于检测的α-羟基三唑仑和三唑仑成分.同时,通过对人体及动物进行实验,初步确定了三唑仑及α-羟基三唑仑在人体中反动物体内的代谢和动物体内的分布情况.该法具有方法简便、灵敏度高、重现性好、线性范围广、回收率高的特点,经办案实践,证实能够满足实际办案工作的需要. Enzyme hydrolysis using internal quality control, liquid-liquid extraction, GC-ECD and GC-MS / MS detection of in vivo tissue and body fluids of triazolam and α-hydroxy triazolam can be detected simultaneously Other methods are difficult to detect the α-hydroxy-triazolam and triazolam composition.At the same time, experiments on human beings and animals, preliminary identified triazolam and α-hydroxy-triazolam in the body of the anti-animal body metabolism and animals The method has the advantages of simple method, high sensitivity, good reproducibility, wide linear range and high recovery rate. The practice of this method has proved that it can meet the needs of actual handling work.