论文部分内容阅读
目的:探讨络脉“络气虚滞”动物模型的建立方法,为“络气虚滞”的临床研究和中药新药研究提供理想的动物模型。方法:选用8周龄SD大鼠,采用力竭性跑台运动,并结合基础饮食(5g.100g-1体质量)建立气虚证候模型,同时采用200ng·min-1·kg-1血管紧张素Ⅱ(AngⅡ)(背部埋埴AngⅡ药物缓释泵)损伤血管,建立大鼠脉络“络气虚滞”的病理证候复合模型,补气药物组对所建模型予以验证。实验分成正常组、运动组(基础饮食)、AngⅡ组、复合模型组和药物组5组,每组10只。用放射免疫法测定血液中内皮素(endothelin-1,ET)、去甲肾上腺素(norepinephrine,NE)、AngⅡ、促肾上腺皮质激素(adreno-coticotropic hormone,ACTH)、白介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor,TNF-α)含量,扫描电镜观察主动脉内皮形态的变化。结果:运动组、AngⅡ组、复合模型组均出现内皮损伤,血液中ET、IL-6、TNF-α均有不同程度的升高,以复合模型组改变明显。符合模型组出现气虚症状,内皮细胞充血、水肿、脱落,内弹力板暴露,而ACTH、NE下调,药物组运动耐力明显改善,内皮损伤轻。结论:采用基础饮食、力竭运动及AngⅡ多因素建立络脉“络气虚滞”病理症候复合模型可行,基本符合中医气虚证候特点和现代医学血管损伤病理变化。
OBJECTIVE: To explore the establishment of animal model of collaterals and collaterals stagnation, and to provide an ideal animal model for the clinical study of “colorectal Qi” and the study of new Chinese medicine. Methods: Eight-week-old SD rats were used to establish Qi-deficiency syndrome model by using exhaustive treadmill exercise combined with basal diet (5g.100g-1 body weight), while 200ng · min-1 · kg-1 vascular tension (Ang Ⅱ) (AngⅡ). The compound model of pathological syndromes was established in rats’ venous system with “Qi deficiency and stagnation of blood stasis syndrome”, and the model of gas-filled drug group was validated. The experiment was divided into normal group, exercise group (basal diet), Ang Ⅱ group, compound model group and drug group 5 groups, 10 rats in each group. The levels of endothelin-1, NE, AngⅡ, ACTH, interleukin-6 IL-6) and tumor necrosis factor-α (TNF-α) were detected by scanning electron microscopy. Results: Endothelium injury occurred in both exercise group, Ang Ⅱ group and compound model group. ET, IL-6 and TNF-α levels in blood increased to some extent, and changed obviously in compound model group. Consistent with the model group of symptoms of qi deficiency, endothelial cell congestion, edema, exfoliation, endometrial exposure, and ACTH, NE down significantly improved exercise tolerance of drug group, endothelial damage. Conclusion: It is feasible to establish compound model of pathological syndrome of collaterals and collaterals by using multiple factors based on basic diet, exhaustive exercise and angiotensin Ⅱ. It basically conforms to the characteristics of syndrome of traditional Chinese medicine and the pathological changes of vascular injury in modern medicine.