论文部分内容阅读
目的：应用人体骨骼肌 Ｇ Ｐ Ｔ、 Ａ Ｌ Ｐ 和 Ｇｌｕ 检查确定常温下（１８℃）骨骼肌出现不可逆变化。方法：每小时取人体骨骼肌同时准备做电镜检查和进行 Ｇ Ｐ Ｔ、 Ａ Ｌ Ｐ、 Ｇｌｕ 的动态观察，电镜下找出从正常到不可逆变性时 Ｇ Ｐ Ｔ、 Ａ Ｌ Ｐ、 Ｇｌｕ 的变化规律。结果：① Ｇ Ｐ Ｔ 在缺血后 ２ ｈ 开始逐渐增高，４ ｈ达高峰，缺血后 ７ ｈ 达到２ ０３５±２４５ Ｕ／ｇ，为电镜下出现不可逆变化。② Ａ Ｌ Ｐ在缺血后 ２ ｈ 为２３±１３ Ｕ／ｇ，４ ｈ 达最高值，７ ｈ 消失，为电镜下不可逆变化。③ Ｇｌｕ ２ ｈ 为２２５±０１５ ｍ ｍ ｏｌ／ｇ，７ ｈ降至 ０７６±００１７ ｍ ｍ ｏｌ／ｇ，为电镜下出现不可逆变化。结论： Ｇ Ｐ Ｔ＜ ２ ０３５±２４５ Ｕ／ｇ， Ａ Ｌ Ｐ消失， Ｇｌｕ＜ ０７６±００１７ ｍ ｍ ｏｌ／ｇ，可判定肌肉出现不可逆变性。
OBJECTIVE: To determine the irreversible changes of skeletal muscle at room temperature (18 ℃) by using human PTE, GLP and Glu tests. Methods: The human skeletal muscle was taken every hour. Electron microscopy and dynamic changes of G P T, A L P and Glu were performed at the same time. The changes of G P T, A L P and Glu during normal and irreversible degeneration were found under electron microscope . Results: ① G P T began to increase at 2 h after ischemia and peaked at 4 h, reached 2 035 ± 24 5 U / g at 7 h after ischemia, which showed an irreversible change under electron microscope. ② A L P at 2 h after ischemia was 23 ± 1 3 U / g, reaching the highest value at 4 h and disappearing at 7 h, which was an irreversible change under electron microscope. ③ Glu 2 h was 2 25 ± 0 15 m m ol / g, and dropped to 0 76 ± 0 017 m m ol / g in 7 h, which showed an irreversible change under electron microscope. CONCLUSION: G P T <2035 ± 24.5 U / g, A L P disappeared, Glu <076 ± 0017 m m ol / g, and the irreversible degeneration of muscle could be judged.