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据固氮酶厦门模型Ⅱ合成的固氮酶铁钼辅基模拟物与缺陷型棕色固氮菌uw-45提取液重组后,在完全的酶促反应条件下,具有明显的生物催化活性,其还原乙炔为乙烯的转化数通常在3—6(分~(-1))之间,相当于天然铁钼辅基活性的1—2％左右;还原分子氮为氨的转化数在1.5—3(分~(-1))左右,可用普通微量氨扩散法和Nessler比色法检出。重组模拟物的乙炔转化数与氮转化数之比值符合于棕色固氮菌活性固氮酶相应的转化数之比。经柠檬酸盐处理后,以氯为外配位基的模拟物的重组活性可显著提高。
According to nitrogenase-Xiamen model II synthesis of nitrogenase enzyme molybdenum cofactor mimics and defective Azotobacter vinelandii uw-45 extract after recombination, in the complete enzymatic reaction conditions, with significant biocatalytic activity, the reduction of acetylene is The conversion of ethylene is usually between 3 and 6 (min -1), corresponding to about 1-2% of the activity of natural iron and molybdenum prosthetic groups. The conversion of nitrogen to ammonia is 1.5-3 (min ~ (-1)) can be detected by ordinary trace ammonia diffusion method and Nessler colorimetric method. The ratio of the number of acetylene conversion to the number of nitrogen conversion in the recombinant mock accorded with the corresponding conversion ratio of Azotobacter vinelandii. After citrate treatment, the recombinant activity of the mimetic with the chlorinated ligand can be significantly increased.