论文部分内容阅读
本文用粒系祖细胞(CFU-GM)及经液体培养一周骨髓细胞基质层,同种骨髓粒系祖细胞(称为 SL-CFU-GM),分别作为检测骨髓造血细胞及造血基质功能的方法。观察活血化瘀药对造血的影响。将小鼠给予川芎嗪、复方丹参液,并以环磷酰胺及生理盐水作对照,取骨髓测定 CFU-GM、SL-CFU-GM。结果活血药均不能直接促进造血细胞增殖。但 SL-CFU-GM 明显高于对照组,加消炎痛后 SL-CFU-GM 更形增多。说明活血药促进基质功能,且与 PGE 有关。提示活血化瘀药在体内有改善骨髓造血微环境,从而促进造血。为临床应用活血药于造血系统疾病,提供了实验基础。
In this article, we used granulocyte-progenitor cells (CFU-GM) and a one-week bone marrow cell stromal layer in liquid culture, and the same type of myeloid granulocyte progenitor cells (called SL-CFU-GM) as a method for detecting the function of bone marrow hematopoietic cells and hematopoietic substrates, respectively. . Observe the effect of blood circulation expectorant on hematopoiesis. The mice were given tetramethylpyrazine, compound Danshen solution, and cyclophosphamide and saline as a control, bone marrow determination of CFU-GM, SL-CFU-GM. Results The blood-activating drugs could not directly promote the proliferation of hematopoietic cells. However, SL-CFU-GM was significantly higher than that of the control group, and SL-CFU-GM increased even more after indomethacin treatment. It shows that blood-activating drugs promote matrix functions and are related to PGE. It is suggested that blood circulation expectorant drugs can improve the hematopoietic microenvironment of bone marrow in the body, thereby promoting hematopoiesis. For the clinical application of blood drugs in hematopoietic system diseases, provided the experimental basis.