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用绒毛细胞作细胞遗传学分析,有两种方法,一是培养法,一是利用细胞滋养层内朗罕氏细胞自发分裂相的直接法。绒毛细胞的长期培养有两个主要缺点:(1)因母体细胞污染可致诊断错误。(2)培养时间长不能及时出报告。伦敦两个中心(ST.Mary′Hospital(SMH))及(King′sCollege Hospital(KCH))共完成225例绒毛培养,其中100例为诊断病例,并对所获结果的可靠性及正确性进行了评价。两个中心均用Heaton(1984)绒毛培养法并作了改进。SMH有30例以张氏培养液代替F10,由于张氏液不稳定,在培养3～5天后要更换。再过3～5天如观察到了3个以上健康、活跃的细胞群落,就以F10代替张氏液。若生长缓慢则继续更换张氏液直到出现活跃的
Villus cells for cytogenetic analysis, there are two ways, one culture method, one is the use of cytotrophoblast Langerhans cells within the spontaneous split phase of the direct method. Long-term culture of villus cells has two major drawbacks: (1) Diagnostic errors due to maternal cell contamination. (2) long training can not be timely report. A total of 225 villus cultures were completed in two centers in London (ST.Mary’Hospital (SMH) and King’s College (KCH)), of which 100 were diagnosed and the reliability and correctness of the results obtained The evaluation. Both centers use Heaton (1984) villi culture and make improvements. There are 30 cases of SMH instead of F10 Chang’s medium, due to unstable Chang, after 3 to 5 days to be replaced. Another 3 ~ 5 days as observed more than 3 healthy and active cell population, to F10 instead of Chang solution. If the slow growth continue to replace Chang fluid until active