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目的:观察重症中暑大鼠脊斜肌微循环血流动力学变化,探索抗氧化剂对重症中暑血流动力学的保护作用。方法:67只Wistar雄性大鼠实验前12h禁食不禁水,取27只分3组:造模组(HS组)、SOD预处理后造模组(SOD+HS组)、NS预处理后造模组(NS+HS组);每组分为3个亚组:对照组、热打击至中心体温为38℃组及热打击至中心体温为41℃组,测量各组ROS水平。取40只大鼠随机分为4组:对照组(Control组)、热打击组(HS组)、SOD预处理后热打击组(SOD+HS组)、NS预处理后热打击组(NS+HS组),活体显微镜下动态观察大鼠脊斜肌内相同三级以下微血管血流速度、血管管径、血流量。结果:随中心体温增高,脊斜肌中ROS水平逐渐增高。热打击后各组大鼠的微动脉及微静脉血流量明显减低。SOD+HS组ROS水平明显低于HS组及NS+HS组。SOD+HS组微血管红细胞流速比HS组及NS+HS组高;SOD+HS组血流量明显高于HS组及NS+HS组。结论:热打击大鼠局部组织ROS水平增高为局部微循环障碍的重要诱因,抗氧化剂SOD通过降低热打击ROS水平增高对局部微循环发挥有效的保护作用。
Objective: To observe the hemodynamic changes of microcirculation in spina bifida in severe heatstroke rats and to explore the protective effect of antioxidants on the hemodynamics of severe heatstroke. Methods: Sixty-seven male Wistar rats were fasted for 12 hours before the experiment. 27 male Wistar rats were divided into 3 groups: model group (HS group), SOD pretreatment model group (SOD + HS group), NS pretreatment (NS + HS group). Each group consisted of 3 subgroups: the control group, hot-hit to the central body temperature of 38 ℃ group and the thermal shock to the central body temperature of 41 ℃ group. The levels of ROS in each group were measured. Forty rats were randomly divided into four groups: Control group, HS group, SOD + HS group, NS + HS group). Under the microscope, the microvessel flow velocity, blood vessel diameter and blood flow in the same level of spine were observed dynamically under microscope. Results: With the increase of central body temperature, the level of ROS in spine and oblique muscle gradually increased. After heat shock, the arterial and venule blood flow of rats in each group was significantly reduced. The level of ROS in SOD + HS group was significantly lower than that in HS group and NS + HS group. The red blood cell velocity of microvessels in SOD + HS group was higher than that in HS group and NS + HS group. The blood flow in SOD + HS group was significantly higher than that in HS group and NS + HS group. CONCLUSION: Increased ROS levels in the local tissues of rats with thermal shock are the important inducing factors of local microcirculation disturbance. The antioxidant SOD can effectively protect the local microcirculation by decreasing the level of ROS.