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应用图像分析法对114例胃粘膜异型增生标本的细胞核 DNA 含量及核形态参数进行了定量分析,同时进行 DNA 含量直方图分型。结果发现,其 DNA 含量直方图呈整倍体型者(Ⅰ、Ⅱ型)73例(64%),非整倍体型者(Ⅲ、Ⅳ型)41例(36%);非整倍体型组的 DNA 相对含量、超二倍体细胞、超四倍体细胞百分数及核平均面积、周长、体积均较整倍体型组高(P<0.01)。对其中44例进行了6～72个月(平均24个月)的随访,21例整倍体型异型增生者中有1例癌变,23例非整倍体型者中有8例癌变,分别占4.8%和34.8%(P<0.01);此外,整倍体型组中有6例病变减轻,而非整倍体型组中仅1例病变减轻(P<0.05)。本文提示,细胞核 DNA 含量的定量分析及倍体分型,对胃癌前病变的临床监测具有实用价值。非整倍体核型的出现是提示细胞具有癌变潜能的重要指标。
Image analysis was used to quantitatively analyze the nuclear DNA content and nuclear morphological parameters of 114 cases of gastric dysplasia, and DNA content histogram classification was performed at the same time. The results showed that histograms of DNA content were ploidy type (I, II type) 73 cases (64%), aneuploid type (III, IV type) 41 cases (36%); aneuploid type group The relative content of DNA, the percentage of superdiploid cells, hypertetraploid cells and the average area, perimeter, and volume of the nucleus were higher than those in the euploid group (P<0.01). Of these, 44 cases were followed up for 6 to 72 months (average 24 months). Among 21 cases of euploid dysplasia, 1 case had canceration, and of 23 cases of aneuploid type, 8 cases had canceration, accounting for 4.8 cases respectively. % and 34.8% (P<0.01); In addition, 6 lesions were reduced in the euploid group and only 1 lesion was reduced in the non-euploid group (P<0.05). This article suggests that quantitative analysis of nuclear DNA content and ploidy typing have practical value in the clinical monitoring of precancerous lesions. The presence of aneuploid karyotypes is an important indicator of the cancer’s potential for transformation.