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目的:建立冬凌草甲素纳米脂质载体药物含量及包封率测定的高效液相色谱法。方法:Kromasil C18柱(4.60mm×150mm,5μm);流动相:甲醇-水(52∶48);流速1.0mL·min-1;检测波长:242nm;进样量:20μL;柱温:25℃。建立超滤离心法测定冬凌草甲素纳米脂质载体的包封率。结果:在此色谱条件下冬凌草甲素与辅料及溶剂峰均得到良好分离,冬凌草甲素在1.0～100μg·mL-1浓度范围内线性关系良好(r=0.9998,n=7),冬凌草甲素纳米脂质载体的包封率为(68.2±0.60)%。结论:该方法准确可靠、简单快速,可用于冬凌草甲素纳米脂质载体药物含量及包封率的测定。
OBJECTIVE: To establish a method for the determination of drug content and entrapment efficiency of oridonin nano-lipid carrier by high performance liquid chromatography. Method: Kromasil C18 column (4.60mm × 150mm, 5μm); mobile phase: methanol-water (52:48); flow rate 1.0mL · min -1; detection wavelength 242nm; injection volume 20μL; . The entrapment efficiency of oridonin nano-lipid carrier was determined by ultrafiltration centrifugation. Results: The results showed that there was a good linear relationship between oridonin and its excipients and solvent peaks under the chromatographic conditions. Oridonin showed good linearity (r = 0.9998, n = 7) in the range of 1.0 ~ 100μg · mL-1 , The encapsulation efficiency of oridonin nano-lipid carrier was (68.2 ± 0.60)%. Conclusion: The method is accurate, reliable, simple and rapid and can be used for the determination of drug content and encapsulation efficiency of oridonin nano-lipid carrier.