论文部分内容阅读
目的 观察黏附分子P-选择素与树突状细胞(DC)在人肾炎肾组织中的表达与分布,探讨P-选择素和DC与肾小管间质病变以及肾功能损害的关系。方法 选择经肾活检和临床资料确诊的不同类型肾炎患者133例,根据小管间质病变分为三组:轻度组63例,中度组44例,重度组26例;10例正常人肾组织作为对照。采用免疫组化法观察肾炎患者肾组织中P-选择素的表达;利用免疫双染与荧光图像分析法,观察CD1a+CD80+DC在肾组织中的分布和改变,并探讨与肾小管间质病变和肾功能损害的关系。结果 肾炎患者肾组织中P-选择素表达增强,在肾小管上皮细胞表达显著高于肾小球、肾间质和肾血管;而在肾小管间质表达于重度组又明显高于轻、中度组,并与肾小管间质病变程度显著相关。CD1a+CD80+DC在肾组织中分布面积、数量和密度均显著增多,且以肾小管间质为主,分布于肾小管、肾间质和肾血管;其在肾小管间质分布于重度组也明显高于轻、中度组,并与肾小管间质病变程度以及肾功能明显相关。此外,CD1a+CD80+DC肾小管间质分布状况亦与P-选择素表达显著相关。件论黏附分子;P-选择紊可能介导DC肾炎肾组织中迁移聚集,参与了肾脏免疫病理机制,并与肾小管间质病变密切相关。
Objective To observe the expression and distribution of adhesion molecule P-selectin and dendritic cells (DCs) in human nephritis and explore the relationship between P-selectin, DC and tubulointerstitial lesions and renal dysfunction. Methods 133 patients with different types of nephritis confirmed by renal biopsy and clinical data were divided into three groups according to tubulointerstitial lesions: 63 in mild group, 44 in moderate group and 26 in severe group, and 10 in normal kidney tissue as comparison. Immunohistochemistry was used to observe the expression of P-selectin in renal tissue of nephritis patients. The distribution and changes of CD1a + CD80 + DC in renal tissues were observed by immunofluorescence double staining and fluorescence image analysis. Relationship between lesions and impaired renal function. Results The expression of P-selectin in renal tissue was increased in glomerulonephritis patients, and was significantly higher in glomeruli, renal interstitium and renal blood vessels in renal tubular epithelial cells than in mild glomerulonephritis Degree group, and with tubulointerstitial lesions was significantly related. The area, number and density of CD1a + CD80 + DC in renal tissue were significantly increased, and mainly in the tubulointerstitial, distributed in the renal tubules, renal interstitium and renal blood vessels; in the tubulointerstitial distribution in the severe group Also significantly higher than mild to moderate group, and tubulointerstitial lesions and renal function was significantly related. In addition, the distribution of CD1a + CD80 + DC tubulointerstitial was also significantly associated with P-selectin expression. P-selectin may mediate the migration and aggregation in renal nephritis, which is involved in renal immune pathology and is closely related to tubulointerstitial lesions.