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目的探讨蝙蝠葛碱对阿尔茨海默病模型小鼠的影响及其机制。方法雄性小鼠随机分成对照组、模型组、吡拉西坦组(0.62g·kg~(-1)·d~(-1))、蝙蝠葛碱组(1.17g·kg~(-1)·d~(-1))。采用Aβ1﹣42(80pmol·μl~(-1))双侧脑室注射小鼠诱导阿尔茨海默病模型。所有小鼠灌胃给予等体积的药物或者生理盐水,每天灌胃1次,持续28d。采用Morris水迷宫测试行为学指标并观察小鼠的学习记忆能力,观察小鼠海马镜下形态结构、脑和脾脏的脏器指数,检测小鼠脑组织含水量,采用双抗体夹心法测定脑组织Aβ和IL-6水平。结果与对照组比较,模型组小鼠潜伏期延长,游泳距离增加(P<0.05),神经元数量减少、变性,脑和脾脏的脏器指数明显降低(P<0.05),脑组织含水量明显升高(P<0.05),Aβ和IL-6水平明显升高(P<0.05);与模型组比较,蝙蝠葛碱能明显改善模型小鼠学习记忆能力(P<0.05),海马神经元细胞形态明显改善,脑和脾脏脏器指数明显升高(P<0.05),脑组织含水量明显降低(P<0.05),Aβ和IL-6水平明显降低(P<0.05)。结论蝙蝠葛碱能改善阿尔茨海默病模型小鼠的学习记忆功能及海马神经元的正常形态结构、脏器指数,蝙蝠葛碱通过抑制Aβ和IL-6蛋白在阿尔茨海默病治疗中发挥重要的作用。
Objective To investigate the effect of dauricine on Alzheimer’s disease in mice and its mechanism. Methods Male mice were randomly divided into control group, model group, piracetam group (0.62g · kg -1 · d -1), dauricine group (1.17g · kg -1) · D ~ (-1)). Aβ1-42 (80 pmol·μl ~ (-1)) bilateral intracerebroventricular injection of mice induced Alzheimer’s disease model. All mice given intragastric administration of equal volume of drugs or saline, gavage once a day for 28 days. Morris water maze test behavioral indicators and learning and memory ability of mice observed morphological changes in mouse hippocampal microscopy, organ index of brain and spleen, detection of mouse brain water content, using double antibody sandwich method for determination of brain tissue Aβ and IL-6 levels. Results Compared with the control group, the mice in the model group had longer latency and longer swimming distance (P <0.05). The number of neurons decreased, degeneration, organ index in brain and spleen decreased significantly (P <0.05), and the water content in brain increased significantly (P <0.05), while the levels of Aβ and IL-6 in model group were significantly higher than those in model group (P <0.05). Compared with model group, dauricine significantly improved learning and memory ability of model mice (P <0.05) (P <0.05). The water content of brain tissue was significantly decreased (P <0.05) and the levels of Aβ and IL-6 were significantly decreased (P <0.05). Conclusion Dauricine improves the learning and memory abilities and the normal morphological structure of hippocampal neurons in Alzheimer’s disease model mice. The daunorubicin treatment of Alzheimer’s disease by inhibiting Aβ and IL-6 protein Play an important role.