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同工酶用于鉴定树种在国外有十余年历史。其鉴定速度和可靠性超过了传统的树木分类法。同工酶分析是基于树木种间生化过程不同,酶谱显示差异的原理进行的,它更能反映树种间的本质差别。辽宁省广泛分布着一种有别于板栗(Castanea mollissima Blume)和日本栗(Castanea crenata Sieb.et Cuce)的杂种栗树类型,对这类的归属,过去争议很大。我们通过种源调查、植物学性状观察,特别是通过过氧化物酶同工酶酶谱的分析,确认该类板栗为自然杂种。一、试验材料供试材料有;引于河北的遵化11号、下庄2号,山东的金丰、郯城207,江苏的处暑红,广西的中果红油皮;和原产于日本的乙吹、银
Isoenzyme used to identify species in foreign countries more than ten years of history. The speed and reliability of its identification exceeds the traditional tree taxonomy. Isoenzyme analysis is based on the principle of different tree biochemical processes, zymograms show differences, it better reflect the essential differences between species. A type of hybrid chestnut tree widely distributed in Liaoning province that is different from Castanea mollissima Blume and Castanea crenata Sieb.et Cuce has been disputed in the past. Through the investigation of provenance and the observation of botanical traits, especially through the analysis of peroxidase isozyme zymogram, we identified this type of chestnut as a natural hybrid. First, the test materials for the test materials are; Cited in Hebei Zunhua 11, Xiazhuang 2, Shandong Jinfeng, Gaocheng 207, Jiangsu Shuzhu Hong, Guangxi, the fruit red oil skin; and B blowing originating in Japan ,silver