论文部分内容阅读
目的 探讨杂色曲霉素(ST)对巨噬细胞免疫功能的影响。方法 分别采用半定量逆转录聚合酶链反应及酶联免疫吸附法,研究0 .12 5 ,0 .2 5 ,0 .5 ,1.0和2 .0mg·L- 1ST预处理对小鼠腹腔巨噬细胞白细胞介素(IL) 12mRNA表达及其蛋白分泌的影响。结果 在0 .12 5~1.0mg·L- 1范围内,ST处理对小鼠腹腔巨噬细胞IL 12p4 0和IL 12p35mRNA的表达均有一定的抑制作用。但在蛋白水平,对IL 12分泌的影响不明显。当ST浓度达到2 .0mg·L- 1时,可明显抑制IL 12p4 0mRNA的表达及IL 12的分泌。结论 ST可抑制巨噬细胞IL 12的表达或分泌,提示ST可能在一定程度上对机体的细胞免疫功能产生不利影响。
Objective To investigate the effect of streptozotocin (ST) on immune function of macrophages. Methods Semiquantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were used to study the effects of 0.125, 0.55, 0.5, 1.0 and 2.0 mg · L- 1ST pretreatment on the peritoneal macrophages Effect of interleukin (IL) 12mRNA expression and protein secretion in. Results In the range of 0.12 5 ~ 1.0 mg · L -1, ST treatment inhibited the expression of IL 12p40 and IL 12p35 mRNA in mouse peritoneal macrophages. But at the protein level, the effect on IL 12 secretion is not obvious. When ST concentration reached 2.0 mg · L -1, the expression of IL 12p40 mRNA and the secretion of IL 12 were significantly inhibited. Conclusion ST can inhibit the expression or secretion of IL-12 in macrophages, suggesting that ST may have an adverse effect on the cellular immune function to a certain extent.